Fig. 4: scRNA-seq demonstrates functional dominance of m.1227G>A rRNA hotspot mutation.
From: Functionally dominant hotspot mutations of mitochondrial ribosomal RNA genes in cancer
a, Single-cell workflow. b, Heteroplasmy distribution of all single-cell libraries. Boxplots indicate median and interquartile range; error bars, S.E.M c, Correlation of MT-CO1 expression with heteroplasmy across HEK293 (Spearman Correlation) and 143B BE1 library (Spearman Correlation). d, Sorted Fisher Z-transformed Spearman correlation ranks of heteroplasmy gene correlates from the 143B BE1 library. e, Association of HEK293 and 143B BE1 library gene ranks with selected genes annotated in red. f, GSEA volcano plot from 143B BE1 heteroplasmy gene correlates, negative normalized enrichment score (blue) represents downregulation with heteroplasmy whereas positive normalized enrichment score (red) represents upregulation with heteroplasmy. P values are adjusted using Benjamini–Hochberg correction.