Extended Data Fig. 7: Impact of siRNA knockdown of ST6GAL1 in A549 cells on influenza infectivity.
From: Genetic risk factors for COVID-19 and influenza are largely distinct
In each plot, bars represent the averages across replicates from each experiment (n = 2), points represent the values from the individual replicates (n = 4 for experiment 1; n = 3 for experiment 2), and lines show the width of the distribution of the data points. The first column shows mRNA levels of ST6GAL1 relative to ACTB transcript in cells treated with four different siRNAs: two targeting ST6GAL1 (siRNA1 and siRNA2) and two negative controls (one targeting GAPDH [experiment 2 only] and a scrambled siRNA). The second and third columns show results from infection assay with PR8-GFP (H1N1, multiplicity of infection [MOI] of 0.4 to 10), with the latter column showing infectivity relative to the scrambled siRNA control. The GAPDH siRNA (but not the two siRNAs against ST6GAL1) significantly reduced GAPDH expression relative to the scrambled siRNA ( ~ 80% reduction). P-values derived from a two-sided Wilcoxon Rank Sum Test and asterisks (*) mark those experiments with P < 0.05.