Extended Data Fig. 1: Testing different Cas proteins for RNA imaging. | Nature Biotechnology

Extended Data Fig. 1: Testing different Cas proteins for RNA imaging.

From: Single-molecule live-cell RNA imaging with CRISPR–Csm

Extended Data Fig. 1

Representative images of GFP-tagged PspCas13b (a), RfxCas13d (b) and Csm complex (c) targeting XIST RNA in HEK293T cells (left), immunostaining for the heterochromatin marker H2AK119ub (middle), and their overlay (right). For each system, a single crRNA targeting the same repetitive sequence in XIST was used. Scale bar, 10 μm.

Back to article page