Extended Data Fig. 8: Monocyte and macrophage lineage development and tissue specialization.
From: Mouse lemur cell atlas informs primate genes, physiology and disease
a. UMAP of atlas monocyte, macrophages, and their progenitors, integrated by FIRM across tissues and individuals (10x and SS2, L1-L4), colored by major cell types (top left), lemur individual (top middle), scRNA-seq method (bottom left), tissue source (bottom middle), and major groups of tissue-specific/resident macrophages (right). Note separate clustering of different macrophage subtypes as well as a unique population of activated monocytes (L2 bladder and perigonadal fat). b. Dot plot showing mean expression of classical marker genes for hematopoietic precursors, granulocyte-monocyte progenitors (GMP), monocytes, macrophage, and tissue-resident macrophage markers across monocyte/macrophage cell types and their progenitors, separated by tissue. Note some markers are shared between multiple cell types (see Supplementary Table 1 in the accompanying paper1). [CD14L], LOC105862649; [CD163], LOC105869074; [CD209L], LOC105885453. c. Dot plot showing mean expression across cell type as in b of top DEGs in the indicated tissue-resident macrophage populations compared to all other macrophage populations. [CD300C], LOC105878881; [HLA-DRB1L], LOC105876782; [HLA-DQA2], LOC105869752; [CCL3L], LOC105882215; [TLL], LOC105872655; [SIGLEC8], LOC105866341; [SIGLEC7], LOC105882132; [FCGR3AL], LOC105873562; [KRT76L], LOC105871481; [PRXL2A], LOC105863040. d. Dot plot showing mean expression of the top DEGs in the population of separately-clustered monocytes from L2 bladder and perigonadal fat compared to the other atlas monocytes/macrophages (blood, lung shown), separated by individual. DEGs include inflammation-associated genes CD274/PD-L1, IL23A, AREG, CSF3, IL1A, suggesting these could be activated populations. [Uncharacterized 1], LOC105869025. See Supplementary Note 7 for further analysis.