Extended Data Fig. 3: Structural determinants of energetic couplings inferred from ddPCA data from combinatorial library 3.

a, Relationship between folding coupling energy strength and minimal inter-residue side-chain heavy-atom distance for combinatorial library 3 (see Fig. 4). The mean is shown and error bars indicate 95% confidence intervals from a Monte Carlo simulation approach (n = 10 experiments). Points are coloured by binned inter-residue distances (see legend in panel b). Spearman’s ρ is shown for all couplings, as well as those involving pairs of residues separated by more than five residues in the primary sequence. Core residues are indicated as triangles. b, Relationship between folding coupling energy strength and linear sequence (backbone) distance in number of residues. The measure of centre and error bars are as defined in panel a.