Extended Data Fig. 3: Calculation of Antp protein eyespot expression diameter.

Size of the Antp protein expression domain in eyespots was calculated as eyespot expression diameter. For each ventral hindwing eyespot (here we use Cu1 eyespot as an example), eyespot expression diameter was calculated as the mean of the two diameters of the Antp protein expression domain (a and b), one (a) was parallel to the proximal-distal axis of the sector (outlined in dotted lines), while the other (b) perpendicular to it. The distance between M3 and Cu1 eyespot centers (c) was constantly used as a hindwing size proxy, and entered as a covariate in statistical analyses. Scale bar: 100 microns.