Extended Data Fig. 1: Vax hydrogel restricted systemic infection in vivo.
From: Immunization against Zika by entrapping live virus in a subcutaneous self-adjuvanting hydrogel
IFNAR1−/− mice were injected with PBS, ZIKV, and Vax; n = 5 for each group at days 5–28, and n = 3 for each group at day 52. a, Viral RNA in the spleens, kidneys, liver, hearts, brains and testes of PBS-injected mice was determined by real-time quantitative RT-PCR. L.O.D., limit of detection. b, The weight of testes from IFNAR1−/− mice after injected with PBS, ZIKV, or Vax. c, Representative H&E staining of the testes from IFNAR1−/− mice after injected with PBS, ZIKV, and Vax. d, Survival curves of IFNAR1−/− mice after injection with PBS, ZIKV or ZIKV loaded Vax; n = 6 for each group. All the mice in the Vax injected group survived compared with the 50% survival rate of the WT ZIKV group. Statistical analyses were performed by using log-rank test.