Table 1 Vaccine egg-adapted mutations in the HA head domain of egg-grown vaccine viruses and CVVs in year 1 and year 2 of the study

45

74

120

164

183

209

223

225

282

295

298

Year 1

A/Michigan/45/2015 cell ^a

R

S

T

S

S

K

Q

G

P

I

I

A/Michigan/45/2015 egg

R

S

T

S

S

K

R

G

P

I

I

A/Michigan/45/2015 X-275 egg CVV (EPI830246) ^b

R

S

T

S

S

M

R

G

P

I

I

A/Singapore/GP1908/2015 IVR-180 egg CVV (EPI848715) ^c,d

R

S

A

S

S

K

Q

A

P

I

I

Year 2

A/Idaho/07/2018 cell ^a,d

R

R

T

T

P

K

Q

G

P

V

I

A/Brisbane/02/2018 egg ^b

G

R

T

T

P

K

R

G

A

V

V

91

121

128

138

142

144

158

159

160

171

190

193

194

225

246

311

Year 1

A/Singapore/INFIMH-16-0019/2016 cell ^a

S

K

T

A

G

S

N(CHO)

Y

T

K

D

F

L

D

N

H

A/Singapore/INFIMH-16-0019/2016 egg

S

K

T

A

G

S

N(CHO-)

Y

K

K

D

F

P

D/G^¶

N

H

A/Singapore/INFIMH-16-0019/2016 IVR-186 egg CVV (EPI1082512) ^b

S

K

T

A

G

S

N(CHO-)

Y

K

K

D

F

P

G

N

H

A/Singapore/INFIMH-16-0019/2016 NIB-104 egg CVV (EPI1151864) ^c

S

K

T

A

G

S

N(CHO-)

Y

K

K

D

F

P

G

N

H

A/North Carolina/04/2016 cell (EPI701956) ^d

S

N

T

A

R

S

N(CHO)

Y

T

K

D

F

L

D

N

H

Year 2

A/Kansas/14/2017 cell ^a,d

N

N

A

S

G

K

N(CHO-)

S

K

N

D

S

L

D

N

Q

A/Kansas/14/2017 egg ^b

N

N

A

S

G

K

N(CHO-)

S

K

N

N

S

L

D

T

Q

129

197

199

Year 1 & 2

B/Colorado/06/2017 cell

G

N

T

B/Colorado/06/2017 egg

G

T(CHO-)

T

B/Maryland/15/2016 BX-69A egg CVV (EPI1061863) ^b,c

D

N(CHO-)

I

B/Maryland/15/2016 cell (CY220998) ^a

D

N

T

B/Iowa/06/2017 cell (EPI1619422) ^d

G

N

T

154

173

197

252

Year 1 & 2

B/Phuket/3073/2013 cell ^a

A

L

N

M

B/Phuket/3073/2013 egg ^b,c

A

L

D(CHO-)

M

B/Singapore/INFTT-16-0610/2016 cell (EPI1843276) ^d

S

Q

N

V