Fig. 2: CHMP2A-KO increases GSC and HNSCC cell lines sensitivity to NK cell-mediated cytotoxicity.

a 4 hour cytotoxicity assay to determine the effect of CHMP2A in GSC resistance to NK cell-mediated cytotoxicity. Two independent sgRNAs were used (sg#2, sg#3). b immunoblot analysis of WT and KO GSC lines KO for CHMP2A using two sgRNAs (sg#2, sg#3). GAPDH serves as a loading control. c 4 hour cytotoxicity assay to determine the effect of CHMP2A in HNSCC-resistance to NK cell-mediated cytotoxicity. d Western blot analysis showing the rescue of KO phenotype. Here we show Cal27 cells expressing WT levels of CHMP2A, no CHMP2A (KO), or complemented CHMP2A (KO+ overexpressed CHMP2A). HEK293 CHMP2A overexpression lysate (OL) was used as a control. The overexpressed CHMP2A is fused with DDK and MYC tags explaining the 4 kDa increase in molecular weight. For this western blot, GAPDH was used as a loading control. e 4 hour cytotoxicity assay to determine the effect of CHMP2A in Cal27 cells expressing WT levels of CHMP2A, no CHMP2A (KO), or complemented CHMP2A (KO+ overexpressed CHMP2A). a, c, e The error bars represent standard error from the mean (±SEM) across n = 3 replicates. Statistical analysis was performed by one-tailed paired Student t test. Representative of n = 3 independent experiments.