Fig. 4: Spatial heterogeneity and cell-cell interactions in the CNS SFTs.

A Proportion of distinct cell types in three spatial samples, categorized by WHO grade 1-3. B Hematoxylin and eosin (H/E) staining of representative regions of different SFT grades and the spatial distribution of SFT cellular states in the corresponding regions as well as the average expression levels of genes from the HALLMARK_HYPOXIA gene set. A total of six samples were included: one grade I, two grade II, and one grade III. Staining was performed in three independent replicates. C Proportion of cell types from the tumor microenvironment in the four molecular subtypes (total sample n = 88). *p < 0.05; **p < 0.01; ***p < 0.001. (two-sided Wilcoxon Rank Sum and Signed Rank tests). Boxplots display the median (center line), interquartile range (box), and whiskers extending to the smallest and largest values within 1.5 × IQR from the lower (Q1) and upper (Q3) quartiles, respectively. D The immune infiltration in different molecular subtypes by EPIC (left) and MCP-counter (right). Two-sided Wilcox test was used to calculate the differences among four subtypes. E Cell-cell communication between different cell types in CNS SFTs deduced from snRNA-seq. Source data are provided as a Source Data file.