Fig. 4: Ex vivo characterisation of the impact of SERT deletion on postnatal GABAergic circuits in S1BF. | Nature Communications

Fig. 4: Ex vivo characterisation of the impact of SERT deletion on postnatal GABAergic circuits in S1BF.

From: Perinatal serotonin signalling dynamically influences the development of cortical GABAergic circuits with consequences for lifelong sensory encoding

Fig. 4

a schematic of interneuron-pyramidal cell (PYR) circuit in L2/3 of S1BF. b Representative minimal optogenetic stimulation data showing interneuron synaptic input onto L2/3 PYRs. Minimal interneuron input onto L2/3 PYRs for c, VIP (one-way ANOVA (F(2, 40) = 0.7044; p = 0.5004); d, Nkx2-1 (one-way ANOVA (F(2, 56) = 2.781; p = 0.071. Pairwise comparisons: WT-Het p = 0.666; *WT-KO p = 0.047); e, SST (one-way ANOVA (F(2, 32) = 4.492; genotype p = 0.0191; Pairwise comparisons: WT-Het p = 0.9733; *WT-KO p = 0.019) interneurons. f Conditional tdTomato expression in thalamic afferents innervating S1BF (P7 SERT-Het pup)(n = 29 Olig3-Cre animals used in experiments); scale bar: 250 μm. g Representative minimum (Min.)/maximum (Max.) optogenetically-evoked thalamocortical (Th-)EPSCs recorded in L4 spiny stellate neurons (SSNs) in WT/SERT-KO pups. h Minimum Th-EPSC recorded in SSNs across the three genotypes (one-way ANOVA (F(2, 48) = 0.6741; p = 0.514). i Min./Max. optogenetically-evoked thalamic feed-forward IPSCs recorded at EGlut in SSNs in WT/SERT-KO pups. j Max. feed-forward IPSC recorded in SSNs across the three genotypes (one-way ANOVA (F(2, 41) = 10.81; p < 0.001). k Schematic of the transient SST input onto SSNs present prior to the onset of PV-mediated feed-forward inhibition. l Total GABAergic input onto SSNs across genotypes during the Th-SERT (P4-6) time window. Dashed white lines, average layer boundaries. m Average profile of GABAergic input onto SSNs recorded in WT and SERT-KO pups. n H-event frequency in WT pups from SERT-WT (n = 12 pups)/Het (n = 17 pups) dams at P7-10. (Mann–Whitney U-test p = 0.277). o GCaMP6s signal amplitude of cells during H-events in WT mice from SERT-WT (n = 12 pups) or SERT-Het (n = 17 pups) dams at P7-10 (t-test p = 0.916). p Pairwise cell correlations across distance in the same pups at P8 (two-way ANOVA, genotype p = 0.790, SERT-WT dam: n = 12 pups; SERT-Het dam: n = 17 pups). q GCaMP6s whisker response across development (two-way ANOVA, genotype p = 0.746, SERT-WT dam: n = 12 pups; SERT-Het dam: n = 17 pups). Box plot whiskers represent minimum to maximum, boxes extend 25th to 75th percentile, centre line is median. Errorbar (p, q) plots are presented as mean ± SEM. Violin plots (n, o) are presented as mean values ± max/min value.

Back to article page