Fig. 6: Sb9 decreases GZMB expression by suppressing TP53 expression in CD8 T cells.

a PCR and qRT-PCR results for the GZMB promoter in CD8 T cells following ChIP using TP53 antibody. Mean± SD, n = 3 biological replicates, data represent two independent experiments. b Representative image of EMSA using probes corresponding to the WT or MUT GZMB promoter together with TP53 protein and TP53 antibody. EMSA was repeated independently three times with similar results. c GZMB mRNA level in CD8 T cells transfected with or without TP53 shRNAs. Mean± SD, n = 3 biological replicates, data represent two independent experiments. d Transcription activity of GZMB analyzed by relative luciferase reporter activity assay in scrambled and TP53 shRNAs-transfected CD8 T cells. Mean± SD, n = 3 biological replicates, data represent two independent experiments. e TP53 mRNA level in CD8 T cells transfected with or without the Sb9 expression vector. f TP53 protein level in CD8 T cells transfected with or without Sb9 expression vector. Mean± SD, n = 3 biological replicates, data represent two independent experiments. Sb9 SerpinB9, GZMB granzyme B, WT wild type, MUT mutation, OE overexpression, EMSA, electrophoretic mobility shift assay, PCR polymerase chain reaction, qRT-PCR quantitative reverse transcription-PCR. All statistical tests were two-sided. Statistical significance was assessed using one-way ANOVA followed by post-hoc Tukey’s test except for (b). Adjustments were made for multiple comparisons. *P < 0.05; **P < 0.01; ****P < 0.0001. Source data are provided as a Source Data file.