Fig. 2 | Cell Death & Differentiation

Fig. 2

From: Trabid inhibits hepatocellular carcinoma growth and metastasis by cleaving RNF8-induced K63 ubiquitination of Twist1

Fig. 2

Trabid significantly inhibits HCC growth and metastasis. a Western blot results demonstrate transfection of lentiviral vectors containing individual shRNA against Trabid (shRNA 1–4) (LV-sh-Trabid) induced significant downregulation of Trabid in HCC cells. ShTrabid-1# produced the best knock down effect on Trabid. b1 Western blot for Trabid in the indicated HCC cells transfected with Trabid or shTrabid or the shRNA-resistant expression construct, TrabidΔ, or their matched controls. The effects of Trabid overexpression (b2) or knockdown (b3) on HCC cell proliferation and foci formation. The effects of Trabid overexpression (c1) or knockdown (c2) on HCC cell foci formation. Representative images of the whole plate are shown in the right. d Indicated HCC cells were injected subcutaneously into six NOD/SCID mice. The growth of xenograft tumors was monitored at different time points. e The volume of MHCC97H orthotopic hepatic tumors was determined at various time points, and representative bioluminescence images or orthotopic tumors are shown

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