Supplementary Figure 5: ARCKiss1 neurons synaptically engage a small number of PVH neurons and do not influence food intake.
From: A rapidly acting glutamatergic ARC→PVH satiety circuit postsynaptically regulated by α-MSH
a, Cre-dependent ChR2 fused to a fluorescent protein (AAV-DIO-ChR2-mCherry) was unilaterally targeted to ARCKiss1 neurons. Images show injection site and terminal field in the PVH of ARCKiss1 neurons. Right, schematic shows connection being tested. Representative traces showing assessment of light-evoked EPSCs recorded from an unidentified PVH (uPVH) neuron. b, Expression of AAV-DIO-hM3Dq-mCherry in ARCKiss1 neurons in Kiss1-Cre mice as determined by mCherry immunolabeling. c, Expression of AAV-DIO-hM3Dq-mCherry (left) and Fos (middle) immunolabeling in ARCKiss1 neurons (right, overlay) in mice sacrificed 90 minutes after CNO administration. d, Effect of CNO/hM3Dq stimulation of ARCKiss1 neurons on dark-cycle food intake. Mice from multiple litters, n = 5 animals. Repeated measures two-way ANOVA: Treatment F(1,4) = 0.8651, P = 0.4050; time F(4,16) = 147.8, P < 0.0001; interaction F(4, 12) = 0.4285, P = 0.7860. Data are presented as mean ± s.e.m.