Supplementary Figure 6: SCENIC results for the human brain single-nuclei data set (Lake et al., 2016).
From: SCENIC: single-cell regulatory network inference and clustering
(a) Binary regulon activity matrix. (b) Three GRN-based subpopulations of human interneurons, with the main DNA motifs and transcription factors defining these groups (NFIX for the VIP interneurons, and ESRRG for the PV interneurons), and the top known markers identified in each (note that the TFs themselves are also up-regulated in the respective clusters). Bottom box: Expression of markers for interneuron subtypes.51,52 In the first plot, interneurons are colored according to the marker with highest Z-score. The remaining plots are colored based on gene expression (grey: no expression, dark red: high expression, yellow: intermediate).