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Preimplantation single cell analyses of dystrophin gene deletions using whole genome amplification

Nature Genetics volume 6, pages 19–23 (1994)Cite this article

Abstract

Primer extension preamplification (PEP) increases the scope and capacity of single cell genetic diagnosis by generating sufficient template to perform multiple subsequent DNA analyses using the polymerase chain reaction. We report the simultaneous analysis of single cells at five commonly deleted dystrophin exons and at the ZFX/ZFY loci. Ninety three percent of PEP reactions with single amniocytes, chorionic villus cells and blastomeres were successful, and a blinded analysis of single lymphoblasts from affected males resulted in 93% diagnostic accuracy, demonstrating its applicability in preimplantation prevention of Duchenne muscular dystrophy. Transfer of unaffected male embryos and improved diagnostic reliability are achieved with the ability to perform replicate multilocus analyses from the same blastomere.

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Authors and Affiliations

  1. Institute for Molecular Genetics, Baylor College of Medicine, Houston, Texas, 77030, USA

    Kristleifur Kristjansson, Samuel S. Chong, Ignatia B. Van den Veyver, Stephanie Subramanian & Mark R. Hughes

  2. Department of Obstetrics and Gynecology, Baylor College of Medicine, Houston, Texas, 77030, USA

    Michael C. Snabes

Authors
  1. Kristleifur Kristjansson
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  2. Samuel S. Chong
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  3. Ignatia B. Van den Veyver
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  4. Stephanie Subramanian
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  5. Michael C. Snabes
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  6. Mark R. Hughes
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Kristjansson, K., Chong, S., Van den Veyver, I. et al. Preimplantation single cell analyses of dystrophin gene deletions using whole genome amplification. Nat Genet 6, 19–23 (1994). https://doi.org/10.1038/ng0194-19

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