Figure 2: Structural comparisons of MHF with histone-fold proteins.
From: The structure of the FANCM–MHF complex reveals physical features for functional assembly
(a) Comparisons of MHF with histones from nucleosome (PDB ID: 1AOI). H2A was in cyan, H2B in red, H3 in salmon and H4 in violet purple. The (b) close-up view highlights the Arg–Asp bidentate pair of MHF1. The (c) clos-eup view shows that MHF2 does not contain the bidentate pair. The (d) close-up view illustrates the difference of α1 helix in MHF1 and H2B. The (e) close-up view reveals the local environment causing the α3 kink of MHF2. (f) Overlay of (MHF1–MHF2)2 with (H3–H4)2. The dimer used for alignment is shown in a surface representation. The left close-up view highlights the α2 helix conformation and the bottom close-up view reveals the hydrophobic interactions. The trajectory of α2 helix was modelled with black dashes. (g) The specific interface of (MHF1–MHF2)2 tetramer. Residues participating in the interactions are shown in magenta sticks for one MHF1 molecule and cyan sticks for the pseudo-symmetry-related MHF1′.