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Carbonate alkalinity induces stress responses and renal and metabolic disorders in Nile tilapia: mitigation by camel whey protein hydrolysate diet

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Abstract

Alkaline stress is a major concern in aquaculture that badly affects the aquatic species’ health and hemostasis. This research investigated the effect of carbonate alkalinity exposure on the gills and kidney organs as important organs for hemostasis, as well as the ameliorative role of camel protein hydrolysates (CPH) as dietary additives against alkaline stress detrimental impacts in Nile tilapia (Oreochromis niloticus). The fish (n = 160) were divided into four groups (G1, G2, G3, and G4), with the control (G1) fed a basal diet, while G2 was fed a basal diet supplemented with 75 g CPH/kg and was reared in freshwater (carbonate alkalinity of 1.4 µmol/L, pH = 7.19). The G3 and G4 were reared in alkaline water (carbonate alkalinity of 23.8 µmol/L, pH = 8.65) and fed the same diets as G1 and G2 for 30 days, respectively. The fish were stocked under a water temperature of 26.4 ± 1.5 °C, and the diets were introduced to the fish three times daily at a rate of 4% of their body weight. The results of this research showed that alkaline exposure increased kidney function parameters (creatinine, urea, and uric acid), glucose, and cortisol levels in the exposed fish. Alkaline exposure reduced the blood electrolytes level (calcium, magnesium, sodium, potassium, and chloride) and branchial antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase, and reduced glutathione) and elevated malondialdehyde level in the exposed fish. Significant downregulation of the branchial expression of Na+/K+ ATPase α-3 subunit (0.17-fold), calcium/calmodulin-dependant protein kinase 1 β (0.23 fold), chloride channel protein 2 (0.38-fold), solute carrier family 12 a 2 (0.33-fold), and solute carrier family 4 a 4 (0.21-fold) was in the fish-reared under carbonate alkalinity stress. Alkaline exposure induced severe histopathological changes in the gills and kidney tissue architecture including inflammatory, circulatory, degenerative, and progressive responses. Supplementation of the Nile tilapia diet with 75 g CPH/kg ameliorated renal function and balanced the blood electrolytes, glucose, and cortisol levels in the alkaline-exposed fish. Modulation of the branchial gene expression profile and improving the gills and kidney microstructure were consequences of feeding on CPH diets during alkaline stress situations. Overall, fortifying the Nile tilapia diets with 75 g CPH/kg helps the fish restore their hemostasis and metabolic status during alkaline stress exposure which enables the sustainable culture of this species in such conditions.

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References

  • Acharya S, Dutta T, Das MK (2005) Physiological and ultrastructural changes in Labeo rohita (Hamilton-Buchanan) fingerlings exposed to sublethal acidic and alkaline pH for long duration. Asian Fish Sci 18(3/4):295

    Google Scholar 

  • Addar L, Bensouici C, Zennia SSA, Haroun SB, Mati A (2019) Antioxidant, tyrosinase and urease inhibitory activities of camel αS-casein and its hydrolysate fractions. Small Rumin Res 173:30–35

    Article  Google Scholar 

  • Al Jamali AH (2023) Re-evaluating eurahaline nature of Nile tilapia, Oreochromisniloticus: a hatchery perspective. Int J Fish Aquat Stud 11:223–231

    Article  Google Scholar 

  • APHA (2005) Standard methods for examination of water and wastewater, 21st edn. American Public Health Association, American Water Works Association, Water Pollution Control Federation, New York, pp 1–8

    Google Scholar 

  • Badr G, Ramadan NK, Sayed LH, Badr BM, Omar HM, Selamoglu Z (2017) Why whey? Camel whey protein as a new dietary approach to the management of free radicals and for the treatment of different health disorders. Iran J Basic Med Sci 20(4):338

    PubMed  PubMed Central  Google Scholar 

  • Bal A, Panda F, Pati SG, Das K, Agrawal PK, Paital B (2021) Modulation of physiological oxidative stress and antioxidant status by abiotic factors especially salinity in aquatic organisms. Comp Biochem Physiol Part C Toxical Pharmacol 241:108971

    CAS  Google Scholar 

  • Birnie-Gauvin K, Costantini D, Cooke SJ, Willmore WG (2017) A comparative and evolutionary approach to oxidative stress in fish: a review. Fish Fish 18(5):928–942

    Article  Google Scholar 

  • Bolner K, Baldisserotto B (2007) Water pH and urinary excretion in silver catfish Rhamdia quelen. J Fish Biol 70(1):50–64

    Article  CAS  Google Scholar 

  • Chen X, Liu S, Ding Q, Teame T, Yang Y, Ran C, Zhang Z, Zhou Z (2023) Research advances in the structure, function, and regulation of the gill barrier in teleost fish. Water Biology Security:100139

  • Cheng Y, Zhao J, Ayisi CL, Cao X (2022) Effects of salinity and alkalinity on fatty acids, free amino acids and related substance anabolic metabolism of Nile tilapia. Aquaculture Fisheries 7(4):389–395

    Article  Google Scholar 

  • Churcher AM, Pujolar JM, Milan M, Hubbard PC, Martins RS, Saraiva JL, Huertas M, Bargelloni L, Patarnello T, Marino IA (2014) Changes in the gene expression profiles of the brains of male European eels (Anguilla anguilla) during sexual maturation. BMC Genomics 15:1–13

    Article  Google Scholar 

  • Ciji A, Akhtar MS (2021) Stress management in aquaculture: a review of dietary interventions. Rev Aquac 13(4):2190–2247

    Article  Google Scholar 

  • Copatti CE, Baldisserotto B, Souza CdF, Monserrat JM, Garcia L (2019) Water pH and hardness alter ATPases and oxidative stress in the gills and kidney of pacu (Piaractus mesopotamicus). Neotropical Ichthyol 17(4):e190032

    Article  Google Scholar 

  • da PaixaoLemos CH, Ribeiro CVDM, de Oliveira CPB, Couto RD, Copatti CE (2018) Effects of interaction between pH and stocking density on the growth, haematological and biochemical responses of Nile tilapia juveniles. Aquaculture 495:62–67

    Article  Google Scholar 

  • Ding L, Liu Y, Wei X, Geng C, Liu W, Han L, Yuan F, Wang P, Sun Y (2023) Effects of saline-alkaline stress on metabolome, biochemical parameters, and histopathology in the kidney of crucian carp (Carassius auratus). Metabolites 13(2):159

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Dou Z, Liu C, Feng X, Xie Y, Yue H, Dong J, Zhao Z, Chen G, Yang J (2022) Camel whey protein (CWP) ameliorates liver injury in type 2 diabetes mellitus rats and insulin resistance (IR) in HepG2 cells via activation of the PI3K/Akt signaling pathway. Food Function 13(1):255–269

    Article  CAS  PubMed  Google Scholar 

  • Du D, Lv W, Su R, Yu C, Jing X, Bai N, Hasi S (2021) Hydrolyzed camel whey protein alleviated heat stress-induced hepatocyte damage by activated Nrf2/HO-1 signaling pathway and inhibited NF-κB/NLRP3 axis. Cell Stress Chaperones 26:387–401

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Elias RJ, McClements DJ, Decker EA (2005) Antioxidant activity of cysteine, tryptophan, and methionine residues in continuous phase β-lactoglobulin in oil-in-water emulsions. J Agric Food Chem 53(26):10248–10253

    Article  CAS  PubMed  Google Scholar 

  • Fossati P, Prencipe L, Berti G (1983) Enzymic creatinine assay: a new colorimetric method based on hydrogen peroxide measurement. Clin Chem 29(8):1494–1496. https://doi.org/10.1093/clinchem/29.8.1494/JClinicalChemistry

    Article  CAS  PubMed  Google Scholar 

  • Gan L, Xu Z, Ma J, Xu C, Wang X, Chen K, Chen L, Li E (2016) Effects of salinity on growth, body composition, muscle fatty acid composition, and antioxidant status of juvenile Nile tilapia Oreochromis niloticus (Linnaeus, 1758). J Appl Ichthyol 32 (2)

  • Ge Q, Wang J, Li J, Li J (2023) Effect of high alkalinity on shrimp gills: histopathological alternations and cell specific responses. Ecotoxicol Environ Saf 256:114902

    Article  CAS  PubMed  Google Scholar 

  • Geletu TT, Zhao J (2023) Genetic resources of Nile tilapia (Oreochromis niloticus Linnaeus, 1758) in its native range and aquaculture. Hydrobiologia 850(10):2425–2445

    Article  Google Scholar 

  • Geng L, Tong G, Jiang H (2016) Xu W (2016) Effect of salinity and alkalinity on luciobarbus capito Gill Na+/K+-ATPase enzyme activity, plasma ion concentration, and osmotic pressure. BioMed Res Int 1:4605839

    Google Scholar 

  • Hamed H, Bellassoued k, El Feki A, Gargouri A, (2019) Evaluation of the hepatoprotective effect of combination between fermented camel milk and Rosmarinus officinalis leaves extract against CCl 4 induced liver toxicity in mice. J Food SciTechnol 56:824–834

    CAS  Google Scholar 

  • Hoyle NT, Merritt JH (1994) Quality of fish protein hydrolysates from herring (Clupea harengus). J Food Sci 59:76–79

    Article  CAS  Google Scholar 

  • Huang S, Li C, Li Z, Duanzhi D, Liu Y, Ran F, Ding J, Yan W, Jia C, Zhang Z (2022) Short-term exposure to 5‰ and 15‰ salinity causes the dynamic changes of the NKA gene, enzyme activities and morphological characteristics in fish tissues of Gymnocypris przewalskii. Aquac Res 53(17):6389–6398

    Article  CAS  Google Scholar 

  • Ibrahim HR, Isono H, Miyata T (2018) Potential antioxidant bioactive peptides from camel milk proteins. Animal Nutrition 4(3):273–280

    Article  PubMed  PubMed Central  Google Scholar 

  • Ibrahim RE, Elshopakey GE, Abd El-Rahman GI, Ahmed AI, Altohamy DE, Zaglool AW, Younis EM, Abdelwarith AA, Davies SJ, Al-Harthi HF (2022) Palliative role of colloidal silver nanoparticles synthetized by moringa against Saprolegnia spp. infection in Nile tilapia: biochemical, immuno-antioxidant response, gene expression, and histopathological investigation. Aquaculture Reports 26:101318

  • Ibrahim RE, Elshopakey GE, Aly MY, Abdelwarith AA, Younis EM, Abd-Elhakim YM, Khamis T, Osman A, Metwally MM, Davies SJ (2024) Camel whey protein hydrolysate diet mitigates alkaline stress–induced biochemical disorders and restores the target of rapamycin, MAPK pathway, and autophagy-related gene expression in Nile tilapia. Aquac Int:1–22

  • Izadi A, Khedmat L, Mojtahedi SY (2019) Nutritional and therapeutic perspectives of camel milk and its protein hydrolysates: a review on versatile biofunctional properties. J Funct Foods 60:103441

    Article  CAS  Google Scholar 

  • Jafar S, Kamal H, Mudgil P, Hassan HM, Maqsood S (2018) Camel whey protein hydrolysates displayed enhanced cholesteryl esterase and lipase inhibitory, anti-hypertensive and anti-haemolytic properties. LWT 98:212–218

    Article  CAS  Google Scholar 

  • Jiang Y, Yuan C, Qi M, Liu Q, Hu Z (2022) The effect of salinity stress on enzyme activities, histology, and transcriptome of silver carp (Hypophthalmichthys molitrix). Biology 11(11):1580

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Katikaneni A, Jelcic M, Gerlach GF, Ma Y, Overholtzer M, Niethammer P (2020) Lipid peroxidation regulates long-range wound detection through 5-lipoxygenase in zebrafish. Nat Cell Biol 22(9):1049–1055

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kaushal SS, Likens GE, Pace ML, Utz RM, Haq S, Gorman J, Grese M (2018) Freshwater salinization syndrome on a continental scale. Proc Natl Acad Sci 115(4):E574–E583

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kim B-M, Rhee J-S, Jeong C-B, Seo JS, Park GS, Lee Y-M, Lee J-S (2014) Heavy metals induce oxidative stress and trigger oxidative stress-mediated heat shock protein (hsp) modulation in the intertidal copepod Tigriopus japonicus. Comp Biochem Physiol Part C Toxicol Pharmacol 166:65–74

    Article  CAS  Google Scholar 

  • Kolesarova A, Slamecka J, Jurcik R, Tataruch F, Lukac N, Kovacik J, Capcarova M, Valent M, Massanyi P (2008) Environmental levels of cadmium, lead and mercury in brown hares and their relation to blood metabolic parameters. Journal of Environmental Science Health, Part A 43(6):646–650

    Article  CAS  Google Scholar 

  • Kovacik A, Arvay J, Tusimova E, Harangozo L, Tvrda E, Zbynovska K, Cupka P, Andrascikova S, Tomas J, Massanyi P (2017) Seasonal variations in the blood concentration of selected heavy metals in sheep and their effects on the biochemical and hematological parameters. Chemosphere 168:365–371

    Article  CAS  PubMed  Google Scholar 

  • Kumar D, Chatli MK, Singh R, Mehta N, Kumar P (2016) Enzymatic hydrolysis of camel milk casein and its antioxidant properties. Dairy Sci Technol 96:391–404

    Article  CAS  Google Scholar 

  • Li M, Kong Y, Wu X, Yin Z, Niu X, Wang G (2021) Dietary α-lipoic acid can alleviate the bioaccumulation, oxidative stress, cell apoptosis, and inflammation induced by lead (Pb) in Channa argus. Fish Shellfish Immunol 119:249–261

    Article  CAS  PubMed  Google Scholar 

  • Liu Y, Yao M, Li S, Wei X, Ding L, Han S, Wang P, Lv B, Chen Z, Sun Y (2022) Integrated application of multi-omics approach and biochemical assays provides insights into physiological responses to saline-alkaline stress in the gills of crucian carp (Carassiusauratus). Sci Total Environ 822:153622

    Article  CAS  PubMed  Google Scholar 

  • Lushchak VI (2011) Environmentally induced oxidative stress in aquatic animals. Aquat Toxicol 101(1):13–30

    Article  CAS  PubMed  Google Scholar 

  • Magbanua TO, Ragaza JA (2024) Selected dietary plant-based proteins for growth and health response of Nile tilapia Oreochromis niloticus. Aquaculture Fisheries 9(1):3–19

    Article  Google Scholar 

  • Marx M, Souza C, Almeida A, Descovi S, Bianchini A, Martos-Sitcha J, Martínez-Rodríguez G, Antoniazzi A, Baldisserotto B (2022) Expression of ion transporters and Na+/K+-ATPase and H+-ATPase activities in the gills and kidney of silver catfish (Rhamdia quelen) exposed to different pHs. Fishes 2022, 7, 261. s Note: MDPI stays neutral with regard to jurisdictional claims in published …,

  • Massanyi P, Stawarz R, Halo M, Formicki G, Lukac N, Cupka P, Schwarcz P, Kovacik A, Tusimova E, Kovacik J (2014) Blood concentration of copper, cadmium, zinc and lead in horses and its relation to hematological and biochemical parameters. Journal of Environmental Science Health, Part A 49(8):973–979

    Article  CAS  Google Scholar 

  • Neiffer DL, Stamper MAJIj (2009) Fish sedation, anesthesia, analgesia, and euthanasia: considerations, methods, and types of drugs. 50 (4):343–360

  • NRC (2011) Nutrient requirements of fish and shrimp. National academies press.

  • Nwizugbo KC, Ogwu MC, Eriyamremu GE, Ahana CM (2023) Alterations in energy metabolism, total protein, uric and nucleic acids in African sharptooth catfish (Clarias gariepinus Burchell) exposed to crude oil and fractions. Chemosphere 316:137778

    Article  CAS  PubMed  Google Scholar 

  • Okasha LA, Abdellatif JI, Abd-Elmegeed OH, Sherif AH (2024) Overview on the role of dietary Spirulina platensis on immune responses against Edwardsiellosis among Oreochromis Niloticus fish farms. BMC Vet Res 20:290

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Osman A, El-Hadary A, Korish AA, AlNafea HM, Alhakbany MA, Awad AA, Abdel-Hamid M (2021) Angiotensin-I converting enzyme inhibition and antioxidant activity of papain-hydrolyzed camel whey protein and its hepato-renal protective effects in thioacetamide-induced toxicity. Foods 10(2):468

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Romero MF (2005) Molecular pathophysiology of SLC4 bicarbonate transporters. Curr Opin Nephrol Hypertens 14(5):495–501

    Article  CAS  PubMed  Google Scholar 

  • Salami M, Moosavi-Movahedi AA, Moosavi-Movahedi F, Ehsani MR, Yousefi R, Farhadi M, Niasari-Naslaji A, Saboury AA, Chobert J-M, Haertlé T (2011) Biological activity of camel milk casein following enzymatic digestion. J Dairy Res 78(4):471–478

    Article  CAS  PubMed  Google Scholar 

  • Saliu J, Oluberu S, Akpoke I, Ukwa UJAJoAS (2017) Cortisol stress response and histopathological alteration index in Clarias gariepinus exposed to sublethal concentrations of Qua Iboe crude oil and rig wash. 42 (1):55–64

  • Schmittgen TD, Livak KJ (2008) Analyzing real-time PCR data by the comparative CT method. Nat Protoc 3(6):1101–1108

    Article  CAS  PubMed  Google Scholar 

  • Sherif AH, Elshenawy AM, Attia AA, Salama SS (2021) Effect of Aflatoxin B1 on farmed Cyprinuscarpio in conjunction with bacterial infection. Egyptian J Aquat Biol Fish 25:465–485

    Article  Google Scholar 

  • Sherif AH, Farag EAH, Mahmoud AE (2024) Temperature fluctuation alters immuno-antioxidant response and enhances the susceptibility of Oreochromisniloticus to Aeromonashydrophila challenge. Aquac Int 32:2171–2184

    Article  CAS  Google Scholar 

  • Shin H-S, An K-W, Kim N-N, Choi C-Y (2010) Antioxidant defenses and physiological changes in olive flounder (Paralichthys olivaceus) in response to oxidative stress induced by elevated water temperature. Korean J Ichthyol 22(1):1–8

    Google Scholar 

  • Suvarna KS, Layton C, Bancroft JD (2018) Bancroft’s theory and practice of histological techniques. Elsevier health sciences,

  • Takvam M, Wood CM, Kryvi H, Nilsen TO (2021) Ion transporters and osmoregulation in the kidney of teleost fishes as a function of salinity. Front Physiol 12:664588

    Article  PubMed  PubMed Central  Google Scholar 

  • Tang C, Lee T (2007) The effect of environmental salinity on the protein expression of Na+/K+-ATPase, Na+/K+/2Cl− cotransporter, cystic fibrosis transmembrane conductance regulator, anion exchanger 1, and chloride channel 3 in gills of a euryhaline teleost, Tetraodon nigroviridis. Comp Biochem Physiol Part a: Mol Int Physiol 147(2):521–528

    Article  CAS  Google Scholar 

  • Trinder P (1969) Determination of blood glucose using 4-amino phenazone as oxygen acceptor. J Clin Pathol 22(2):246

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wen B, Jin S-R, Chen Z-Z, Gao J-Z (2018) Physiological responses to cold stress in the gills of discus fish (Symphysodon aequifasciatus) revealed by conventional biochemical assays and GC-TOF-MS metabolomics. Sci Total Environ 640:1372–1381

    Article  PubMed  Google Scholar 

  • Wilkie MP, Wood CM (1996) The adaptations of fish to extremely alkaline environments. Comp Biochem Physiol Part b: Biochem Mol Biol 113(4):665–673

    Article  Google Scholar 

  • Yao Z, Lai Q, Zhou K, Rizalita RE, Wang H (2010) Developmental biology of medaka fish (Oryzias latipes) exposed to alkalinity stress. J Appl Ichthyol 26(3):397–402

    Article  CAS  Google Scholar 

  • Zhang Y, Wen H, Liu Y, Qi X, Sun D, Zhang C, Zhang K, Zhang M, Li J, Li Y (2023) Gill histological and transcriptomic analysis provides insights into the response of spotted sea bass (Lateolabrax maculatus) to alkalinity stress. Aquaculture 563:738945

    Article  CAS  Google Scholar 

  • Zhao Y, Zhang C, Zhou H, Song L, Wang J, Zhao J (2020) Transcriptome changes for Nile tilapia (Oreochromis niloticus) in response to alkalinity stress. Comparative Biochemistry Physiology Part d: Genomics Proteomics 33:100651

    CAS  PubMed  Google Scholar 

  • Zhou H, Yao T, Zhang T, Sun M, Ning Z, Chen Y, Mu W (2024) Effects of chronic saline-alkaline stress on gill, liver and intestinal histology, biochemical, and immune indexes in Amur minnow (Phoxinus lagowskii). Aquaculture 579:740153

    Article  CAS  Google Scholar 

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Funding

This work was supported by the Researches Supporting Project (RSPD2025R700), King Saud University, Riyadh, Saudi Arabia.

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Authors and Affiliations

  1. Department of Aquatic Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44519, Egypt

    Rowida E. Ibrahim

  2. Department of Zoology, College of Science, King Saud University, PO Box 2455, 11451, Riyadh, Saudi Arabia

    Abdelwahab A. Abdelwarith & Elsayed M. Younis

  3. Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44519, Egypt

    Amany Abdel-Rahman Mohamed & Yasmina M. Abd-Elhakim

  4. Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44511, Egypt

    Tarek Khamis

  5. Biochemistry Department, Faculty of Agriculture, Zagazig University, Zagazig, 44511, Egypt

    Ali Osman

  6. Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, King Salman International University, Ras Sidr, Egypt

    Mohamed M. M. Metwally

  7. Department of Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44519, Egypt

    Mohamed M. M. Metwally

  8. Aquaculture Nutrition Research Unit ANRU, Carna Research Station, Ryan Institute, College of Science and Engineering, University of Galway, Galway, H91V8Y1, Ireland

    Simon J. Davies

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  1. Rowida E. Ibrahim
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  2. Abdelwahab A. Abdelwarith
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  9. Yasmina M. Abd-Elhakim
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Contributions

R. E.I., A.A.A., E.M.Y., A.A.M., T. K., A.O., M. M. M. M., S.J. D., and Y.M. A: conceptualization, data curation, formal analysis, investigation, methodology, resources, validation, visualization, and writing review and editing. REI: Writing original draft. All authors read, reviewed, and approved the final manuscript.

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Correspondence to Rowida E. Ibrahim, Amany Abdel-Rahman Mohamed or Yasmina M. Abd-Elhakim.

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The Institutional Animal Care and Use Committee of Zagazig University, Egypt, approved the experimental protocol (ZU-IACUC/2/F/393/2023), and all applicable institutional standards were followed when caring for and using animals in this study.

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Ibrahim, R.E., Abdelwarith, A.A., Younis, E.M. et al. Carbonate alkalinity induces stress responses and renal and metabolic disorders in Nile tilapia: mitigation by camel whey protein hydrolysate diet. Fish Physiol Biochem 51, 66 (2025). https://doi.org/10.1007/s10695-024-01442-2

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